ELISA

Download this guide for tips on choosing the most appropriate antibodies and buffers, maintaining sample quality, washing techniques, detecting multiple targets and monitoring assay performance.

Favored for its simplicity, specificity and cost-effectiveness, ELISA is a useful technique used in many laboratories globally. Here are some tips that I would like to share in the hopes of saving you time, stress and money, and helping you to achieve the best results possible!

Antibodies are immunological proteins that play fundamental roles in host defense against infectious agents such as viruses, bacteria and fungi. Two types of antibodies – polyclonal and monoclonal – provide research scientists with distinct ways to detect or quantify target antigens largely due to differences in specificity and affinity. In this article, we describe polyclonal and monoclonal antibodies in more detail and consider how each type may be used for various applications.

Antibodies are a vital part of the immune system, playing a key role in our ability to fight infection and in the efficacy of vaccinations. However, that’s not where their utility ends. Find out about what antibodies are, where they come from, how they function and how science is putting them to work in the laboratory.